Coding

Part:BBa_K1128000:Design

Designed by: Daniel Cabrera   Group: iGEM13_Penn   (2013-08-29)

CPG DNA methylase mssSI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 484
    Illegal BsaI.rc site found at 365
    Illegal SapI site found at 568


Design Notes

The part has been modified to be biobrick compatible.


Source

This is a modified version of the CPG DNA methylase mssSI native to Spiroplasma sp. (strain MQ-1).


References

Paul Renbaum, Aharon Razin, Mode of action of the Spiroplasma CpG methylase M.SssI, FEBS Letters, Volume 313, Issue 3, 30 November 1992, Pages 243-247, ISSN 0014-5793, http://dx.doi.org/10.1016/0014-5793(92)81201-V. (http://www.sciencedirect.com/science/article/pii/001457939281201V) Keywords: Processive methylation; M.SssI methylase; M.Hpall methylase; M.Hhal methylase